A PCR-Based Method for the Detectionof Streptococcus agalactiae in Milk
نویسندگان
چکیده
منابع مشابه
DETECTION OF S. AGALACTIAE FROM MILK BY PCR Evaluation of Streptococcus agalactiae detection by PCR in Milk and its comparison to other microbiological methods
Background and objectives: The development of molecular biological techniques provide a method for direct detection and identification of pathogens. This study attempted to compare bacterial culture and PCR to detect S. agalactiae in cattle milk samples. The PCR method was based on using primers V1 and V2 derived from the 16s rRNA gene. Materials and Methods: One hundred milk samples were colle...
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Group B Streptococcus (GBS) is an encapsulated, gram-positive pathogen that is an important cause of neonatal invasive infections, including sepsis and meningitis. There are ten known GBS serotypes based on distinct capsule compositions (Ia, Ib, II-IX), and current candidate capsular polysaccharide conjugate vaccines target only a subset of these. Serotyping of GBS isolates is important for und...
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Streptococcus (Strep.) agalactiae is one of the major pathogens of bovine mastitis and is the main cause of subclinical infection. This study attempted to develop a rapid PCR diagnosis procedure using milk samples collected on filter paper disks. Chromatographic filter paper was employed as the preservation media and kept at room temperature for one to four weeks. The revival rate of Strep. aga...
متن کاملevaluation of streptococcus agalactiae detection by pcr in milk and its comparison to other microbiological methods
background and objectives: the development of molecular biological techniques provide a method for direct detection and identification of pathogens. this study attempted to compare bacterial culture and pcr to detect s. agalactiae in cattle milk samples. the pcr method was based on using primers v1 and v2 derived from the 16s rrna gene. materials and methods: one hundred milk samples were colle...
متن کاملStreptococcus agalactiae
Streptococcus agalactiae, a unique bacterium commonly referred to as group B streptococcus (GBS), is evolving to become a major pathogen that causes a wide variety of problems across a broad spectrum of individuals. The greatest controversy over this bacterium involves the obstetrical patient. Lawyers have made this organism a centerpiece, leading their clients to believe that the presence of G...
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ژورنال
عنوان ژورنال: Journal of Dairy Science
سال: 2002
ISSN: 0022-0302
DOI: 10.3168/jds.s0022-0302(02)74245-8